Methods overview

  • Fluorescence Intensity
  • UV/Vis Absorbance Spectra
  • HPLC-DAD  / DAD-MS-MS
  • LC/MS-MS
  • MS ionization  ESI, APCI, APPI
  • Capillary electrophoresis
  • CE detection   UV, Fluorescence, MS possibly

HPLC / HPLC-MS-MS   methods development and work routines optimize

Metabolites ID

Pharmakokinetics     small molecules, peptides

Sphingolipids            sphingosine , sphingosine-1-phosphate

Ceramide and ceramide-1-phosphate        C12, C14, C16, C18:1, C20, C22, C24, C24:1

Prostaglandins

Bile acids

Fatty acids  

Biological amines